ECTRIMS eLearning

High-dimensional cytometry for immunophenotyping
ECTRIMS Learn. Becher B. 10/11/18; 231933; 184
Burkhard Becher
Burkhard Becher
Contributions
Abstract

Abstract: 184

Type: Hot Topic

Abstract Category: N/A

In the early days of flow cytometry, the use of two to three independent parameters to describe and characterize cellular populations was sufficient to estimate the relative proportions of the major leukocyte populations in the blood. The increasing number of parameters which can be measured simultaneously allow for the concomitant characterization of phenotypic and functional properties at the single cell level and led to major discoveries including new cell types. The emergence of cytometers capable of measuring more than 25 parameters simultaneously brings about a new area of cytometry, where intelligent algorithms and computer-aided analysis allow for a thus far unprecedented view onto the single cell proteome. I will discuss recent advances in single cell cytometry and how we apply this technology to immunophenotype MS patients for the identification of disease-relevant signatures for diagnosis, patient stratification and therapy responses.
Disclosure: I have no COI

Abstract: 184

Type: Hot Topic

Abstract Category: N/A

In the early days of flow cytometry, the use of two to three independent parameters to describe and characterize cellular populations was sufficient to estimate the relative proportions of the major leukocyte populations in the blood. The increasing number of parameters which can be measured simultaneously allow for the concomitant characterization of phenotypic and functional properties at the single cell level and led to major discoveries including new cell types. The emergence of cytometers capable of measuring more than 25 parameters simultaneously brings about a new area of cytometry, where intelligent algorithms and computer-aided analysis allow for a thus far unprecedented view onto the single cell proteome. I will discuss recent advances in single cell cytometry and how we apply this technology to immunophenotype MS patients for the identification of disease-relevant signatures for diagnosis, patient stratification and therapy responses.
Disclosure: I have no COI

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