Contributions
Abstract: EP1322
Type: Poster Sessions
Abstract Category: Clinical aspects of MS - Paediatric MS
Background: Clinically isolated syndrome (CIS) is the first demyelinating episode that may or may not result in definitive diagnosis of multiple sclerosis (MS). All cellular components of immunity (T and B cells, monocytes and also neutrophils) have been recently shown to play role in MS pathogenesis. The chemokine system ensures migration and immune cells interactions to the site of inflammation and thus is beyond the scope of investigation. Important one is the chemokine for B cells attraction (CXCL13) that has been elevated in many studies. There are various data about chemoattractants for T cells (CXCL10), monocytes (MCP1) and neutrophils (CXCL8). These data from paediatric MS population are limited.
Objectives: We measured chemokines concentration in cerebrospinal fluid (CSF) of paediatric MS patients and compared them to paediatric patients with CIS and controls.
Methods: Luminex multiple bead technology and software were used to determine the levels of CXCL8, 10, 13 and MCP1 in CSF of 1) 17 MS patients (age: median 16 years, range 12-18 y; female: male =12:5), 2) 25 CIS patients (age: median 13 years, range 4-17 y; female: male = 15:10) and 3) 37 controls (age: median 11 years, range 2-18 y; female: male = 23:14). Controls represented patients with different non-inflammatory neurological conditions. Non-parametric test were used, a P value < 0.05 was considered to be significant.
Results: CSF CXCL8 concentration was significantly increased in MS and CIS patients compared to controls. There was no difference between CSF CXCL8 levels in MS and CIS patients. CSF CXCL10 concentration was increased in MS but not in CIS patients compared to controls. CSF CXCL13 concentration was significantly increased in both MS and CIS patients in comparison with controls and that was most prominent in MS patients. CSF MCP1 concentration was increased in CIS but not in MS patients compared to controls.
Conclusion: Differences in CSF chemokines levels within MS and CIS patients and controls indicated engagement of possibly different immune populations during CIS and MS course. The CNS attraction of B and T cells was more prominent in MS then in CIS patients. The attraction of monocytes was observed only in CIS patients, while the attraction of neutrophils was similar in both MS and CIS patients. Our data supported the importance of further investigation of chemokines levels and their association with the pathogenesis and prognosis of demyelinating disorders.
Disclosure: The authors declare that there are no conflicts of interest.
Abstract: EP1322
Type: Poster Sessions
Abstract Category: Clinical aspects of MS - Paediatric MS
Background: Clinically isolated syndrome (CIS) is the first demyelinating episode that may or may not result in definitive diagnosis of multiple sclerosis (MS). All cellular components of immunity (T and B cells, monocytes and also neutrophils) have been recently shown to play role in MS pathogenesis. The chemokine system ensures migration and immune cells interactions to the site of inflammation and thus is beyond the scope of investigation. Important one is the chemokine for B cells attraction (CXCL13) that has been elevated in many studies. There are various data about chemoattractants for T cells (CXCL10), monocytes (MCP1) and neutrophils (CXCL8). These data from paediatric MS population are limited.
Objectives: We measured chemokines concentration in cerebrospinal fluid (CSF) of paediatric MS patients and compared them to paediatric patients with CIS and controls.
Methods: Luminex multiple bead technology and software were used to determine the levels of CXCL8, 10, 13 and MCP1 in CSF of 1) 17 MS patients (age: median 16 years, range 12-18 y; female: male =12:5), 2) 25 CIS patients (age: median 13 years, range 4-17 y; female: male = 15:10) and 3) 37 controls (age: median 11 years, range 2-18 y; female: male = 23:14). Controls represented patients with different non-inflammatory neurological conditions. Non-parametric test were used, a P value < 0.05 was considered to be significant.
Results: CSF CXCL8 concentration was significantly increased in MS and CIS patients compared to controls. There was no difference between CSF CXCL8 levels in MS and CIS patients. CSF CXCL10 concentration was increased in MS but not in CIS patients compared to controls. CSF CXCL13 concentration was significantly increased in both MS and CIS patients in comparison with controls and that was most prominent in MS patients. CSF MCP1 concentration was increased in CIS but not in MS patients compared to controls.
Conclusion: Differences in CSF chemokines levels within MS and CIS patients and controls indicated engagement of possibly different immune populations during CIS and MS course. The CNS attraction of B and T cells was more prominent in MS then in CIS patients. The attraction of monocytes was observed only in CIS patients, while the attraction of neutrophils was similar in both MS and CIS patients. Our data supported the importance of further investigation of chemokines levels and their association with the pathogenesis and prognosis of demyelinating disorders.
Disclosure: The authors declare that there are no conflicts of interest.