Contributions
Abstract: P1186
Type: Poster Sessions
Abstract Category: Pathology and pathogenesis of MS - Biomarkers
Introduction: The intrathecal immune response in Multiple Sclerosis (MS) has been used for the development of biochemical assays in cerebrospinal fluid (CSF). They included: Link index (which measures IgG), detection of oligoclonal bands (OCB) and recently the kappa free light chain (KFLC) index.
Aim: To verify whether a kappa-chain oriented response is typical of MS.
Methods: this preliminary study enrolled 131 patients: 45 MS, 57 non-inflammatory neurological disease (NID), 29 neurological ID other than MS. KFLC, lambda (L) FLC and IgG were measured in serum and CSF by nephelometry. OCB were detected using isoelectrofocusing (IEF) and categorized as OCB positive (types II and III) or OCB negative (I, IV and V).
Results: In serum, KFLC/LFLC ratio was 0.92±0.43 in OCB positive, not different from OCB negative (1.04±0.93), whereas in CSF the ratio was 14.5±38.20 in type II+III, significantly higher (p< 0.0001) than in type I+IV+V (0.95±0.72). In serum, KFLC/LFLC ratio was 0.98±0.51 in MS, not different from NID (0.81±0.29) and ID (1.23±0.66) whereas in CSF the ratio was 12.7±21.7 in MS, significantly (p< 0.001) higher than NID (0.84±0.58) and ID (1.65±1.84). The IgG/KFLC ratio serum in MS was 809±270, not different from NID (687±237) and ID (778±369). The IgG/KFLC ratio CSF in MS was 23±25 significantly lower (p< 0.001) than NID (151±92) and ID (128±98). The IgG/LFLC ratio serum in MS was 737±366, not different from NID (618±235) and ID (708±536). The IgG/LFLC ratio CSF in MS was 96±108, not different from NID (101±54) and ID (148±123). The KFLC index (ratio between KFLC and albumin quotients) was markedly (p< 0.0001) higher in MS (80±96) compared to NID (4.6±9.0) and ID (13.1±25). The LFLC index (ratio between LFLC and albumin quotients) was only slightly higher (p=0.017) in MS (16.4±17.7) compared to NID (3.7±4.04) and not statistically different compared to ID (6.9±15).
Conclusion: a kappa-chain oriented immune response occurs intratechally in MS, and is associated with the occurrence of OCB bands in CSF and confirms a more powerful diagnostic value of KFLC index compared to LFLC index.
Disclosure: Dr. Vecchio : supported by a Merk-Serono fellowship.
Dr Serino: nothing to disclose.
Dr Saliva: : nothing to disclose.
Dr Manzini : nothing to disclose.
Dr Campisi : nothing to disclose.
Dr Sulas: : nothing to disclose.
Dr Comi: : nothing to disclose.
Prof Cantello : nothing to disclose.
Prof Bellomo: nothing to disclose.
Dr Crespi: nothing to disclose.
Abstract: P1186
Type: Poster Sessions
Abstract Category: Pathology and pathogenesis of MS - Biomarkers
Introduction: The intrathecal immune response in Multiple Sclerosis (MS) has been used for the development of biochemical assays in cerebrospinal fluid (CSF). They included: Link index (which measures IgG), detection of oligoclonal bands (OCB) and recently the kappa free light chain (KFLC) index.
Aim: To verify whether a kappa-chain oriented response is typical of MS.
Methods: this preliminary study enrolled 131 patients: 45 MS, 57 non-inflammatory neurological disease (NID), 29 neurological ID other than MS. KFLC, lambda (L) FLC and IgG were measured in serum and CSF by nephelometry. OCB were detected using isoelectrofocusing (IEF) and categorized as OCB positive (types II and III) or OCB negative (I, IV and V).
Results: In serum, KFLC/LFLC ratio was 0.92±0.43 in OCB positive, not different from OCB negative (1.04±0.93), whereas in CSF the ratio was 14.5±38.20 in type II+III, significantly higher (p< 0.0001) than in type I+IV+V (0.95±0.72). In serum, KFLC/LFLC ratio was 0.98±0.51 in MS, not different from NID (0.81±0.29) and ID (1.23±0.66) whereas in CSF the ratio was 12.7±21.7 in MS, significantly (p< 0.001) higher than NID (0.84±0.58) and ID (1.65±1.84). The IgG/KFLC ratio serum in MS was 809±270, not different from NID (687±237) and ID (778±369). The IgG/KFLC ratio CSF in MS was 23±25 significantly lower (p< 0.001) than NID (151±92) and ID (128±98). The IgG/LFLC ratio serum in MS was 737±366, not different from NID (618±235) and ID (708±536). The IgG/LFLC ratio CSF in MS was 96±108, not different from NID (101±54) and ID (148±123). The KFLC index (ratio between KFLC and albumin quotients) was markedly (p< 0.0001) higher in MS (80±96) compared to NID (4.6±9.0) and ID (13.1±25). The LFLC index (ratio between LFLC and albumin quotients) was only slightly higher (p=0.017) in MS (16.4±17.7) compared to NID (3.7±4.04) and not statistically different compared to ID (6.9±15).
Conclusion: a kappa-chain oriented immune response occurs intratechally in MS, and is associated with the occurrence of OCB bands in CSF and confirms a more powerful diagnostic value of KFLC index compared to LFLC index.
Disclosure: Dr. Vecchio : supported by a Merk-Serono fellowship.
Dr Serino: nothing to disclose.
Dr Saliva: : nothing to disclose.
Dr Manzini : nothing to disclose.
Dr Campisi : nothing to disclose.
Dr Sulas: : nothing to disclose.
Dr Comi: : nothing to disclose.
Prof Cantello : nothing to disclose.
Prof Bellomo: nothing to disclose.
Dr Crespi: nothing to disclose.