Contributions
Abstract: P1118
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - 25 Biomarkers
Background: The detection of oligoclonal bands (OCBs) restricted to the cerebrospinal fluid (CSF) indicates an intrathecal immunoglobulin synthesis which is a sign for a chronic inflammatory brain disease. Isoelectric focusing (IEF) followed by silverstaining or an immunoblot is routinely used for the analysis of OCBs. Emplying these methods IgG-OCBs are found positive in around 95% of patients with multiple sclerosis (MS).
IgM-OCBs, which are challenging to analyse, have been shown to be associated with a worse disease course, especially in patients with a progressive MS form.
We pursued the aim of elucidating whether in the 5% of MS patients with negative IgG OCBs, intrathecal IgG production is truly absent or IgG-bands have been missed due to insufficient sensitivity of the applied routine detection method. For this purpose we used our new, nanoscale capillary isoelectric focusing (CIEF) immunoassay method for the detection of CSF and serum IgGs in a cohort of patients initially diagnosed IgG-OCB negative.
In addition, we used the IEF and CIEF method to measure IgM-OCBs in control and MS patients.
Methods: We used IEF and CIEF followed by immunological detection for the analysis of intrathecal IgG and IgM synthesis.
Results: Ten out of 33 (30%) clinically definite MS patients initially diagnosed OCB-negative became OCB-positive applying the CIEF immunoassay method, whereas only three out of 100 patients with a non-inflammatory neurological disease were not detected OCB-negative (3%).
The measurement of IgM in CSF and serum using IEF and CIEF with subsequent immunological readout could be established. First experiments also show that both methods yield comparable results.
Discussion and conclusion: We could show that new methods for the detection of OCBs, like the nanoscale CIEF approach, seem to be able to increase the diagnostic sensitivity of IgG-OCBs in patients with MS who are initially diagnosed OCB-negative. Additionally, we could detect IgM-OCBs using the standard IEF and the sensitive CIEF immunoassay which is the basis for future experiments with larger cohorts.
Disclosure:
Steffen Halbgebauer: nothing to disclose
Andre Huss: nothing to disclose
Mathias Buttmann: nothing to disclose
Isabel Brecht: nothing to disclose
Andreas Weishaupt: nothing to disclose
Luisa M. Villar: nothing to disclose
Hayrettin Tumani: nothing to disclose
Markus Otto: nothing to disclose
Abstract: P1118
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - 25 Biomarkers
Background: The detection of oligoclonal bands (OCBs) restricted to the cerebrospinal fluid (CSF) indicates an intrathecal immunoglobulin synthesis which is a sign for a chronic inflammatory brain disease. Isoelectric focusing (IEF) followed by silverstaining or an immunoblot is routinely used for the analysis of OCBs. Emplying these methods IgG-OCBs are found positive in around 95% of patients with multiple sclerosis (MS).
IgM-OCBs, which are challenging to analyse, have been shown to be associated with a worse disease course, especially in patients with a progressive MS form.
We pursued the aim of elucidating whether in the 5% of MS patients with negative IgG OCBs, intrathecal IgG production is truly absent or IgG-bands have been missed due to insufficient sensitivity of the applied routine detection method. For this purpose we used our new, nanoscale capillary isoelectric focusing (CIEF) immunoassay method for the detection of CSF and serum IgGs in a cohort of patients initially diagnosed IgG-OCB negative.
In addition, we used the IEF and CIEF method to measure IgM-OCBs in control and MS patients.
Methods: We used IEF and CIEF followed by immunological detection for the analysis of intrathecal IgG and IgM synthesis.
Results: Ten out of 33 (30%) clinically definite MS patients initially diagnosed OCB-negative became OCB-positive applying the CIEF immunoassay method, whereas only three out of 100 patients with a non-inflammatory neurological disease were not detected OCB-negative (3%).
The measurement of IgM in CSF and serum using IEF and CIEF with subsequent immunological readout could be established. First experiments also show that both methods yield comparable results.
Discussion and conclusion: We could show that new methods for the detection of OCBs, like the nanoscale CIEF approach, seem to be able to increase the diagnostic sensitivity of IgG-OCBs in patients with MS who are initially diagnosed OCB-negative. Additionally, we could detect IgM-OCBs using the standard IEF and the sensitive CIEF immunoassay which is the basis for future experiments with larger cohorts.
Disclosure:
Steffen Halbgebauer: nothing to disclose
Andre Huss: nothing to disclose
Mathias Buttmann: nothing to disclose
Isabel Brecht: nothing to disclose
Andreas Weishaupt: nothing to disclose
Luisa M. Villar: nothing to disclose
Hayrettin Tumani: nothing to disclose
Markus Otto: nothing to disclose