Contributions
Abstract: P983
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Background: Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS). The pathogenesis of MS involves both humoral and cellular immunity, with production of a large number of T cell derived cytokines.
Objectives: To analyze the cytokine profile of cerebrospinal fluid (CSF) T cells in patients with relapsing-remitting multiple sclerosis (RRMS) and age matched controls
Methods: CSF samples were collected from 34 untreated RRMS patients and 20 age-matched controls. Nineteen of the 34 RRMS were experiencing a relapse at the time of lumbar puncture. CSF T cells were expanded in medium containing IL-2 and phytohemagglutinin for 10 days. Subsequently, cells were stimulated with PMA/ionomycin and intracellular production of IL-17A, IL-17F, IFN-Υ, GM-CSF, IL-22, TNF-α, IL-4, IL-5, IL-10, IL-13, IL-2 and IL-21 was analyzed by flow cytometry.
Results: Percentages of IL-17A, IL-17A/IL-22 and IL-17A/GM-CSF producing T cells were significantly higher in RRMS patients compared to controls (p< 0.05). Percentages of T cells producing IFN-γ were lower in RRMS patients (p< 0.05). Patients in relapse showed higher percentages of CD4+ T cells producing IL-5, GM-CSF and IL-13 (p=0.05) and lower percentages of CD8+ T cells producing IL-2 (p< 0.05) compared to patients not in relapse. We found a positive correlation between percentages of T cells making IL-13 and the EDSS (r=0.5; p< 0.05) in RRMS patients.
Conclusions: Differences in IL-17, IL-22, GM-CSF and IFN-γ production by CSF cells in RRMS vs. control subjects were observed in this study. Notably, we observed a positive correlation between percentage of T cells producing IL-13 and EDSS in RRMS patients.
Disclosure:
AHC was supported in part by the Manny & Rosalyn Rosenthal.
Dr. John L. Trotter MS Center Chair in Neuroimmunology of the Barnes-Jewish Hospital Foundation.
LP was supported by the Harry Weaver Neuroscience Scholar of the National Multiple Sclerosis Society (NMSS, JF 2144A2/1).
CC was supported during the course of this study by a FISM fellowship (2012/B/1) and subsequently by a NMSS fellowship (FG 2010-A1/2).
Abstract: P983
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Background: Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS). The pathogenesis of MS involves both humoral and cellular immunity, with production of a large number of T cell derived cytokines.
Objectives: To analyze the cytokine profile of cerebrospinal fluid (CSF) T cells in patients with relapsing-remitting multiple sclerosis (RRMS) and age matched controls
Methods: CSF samples were collected from 34 untreated RRMS patients and 20 age-matched controls. Nineteen of the 34 RRMS were experiencing a relapse at the time of lumbar puncture. CSF T cells were expanded in medium containing IL-2 and phytohemagglutinin for 10 days. Subsequently, cells were stimulated with PMA/ionomycin and intracellular production of IL-17A, IL-17F, IFN-Υ, GM-CSF, IL-22, TNF-α, IL-4, IL-5, IL-10, IL-13, IL-2 and IL-21 was analyzed by flow cytometry.
Results: Percentages of IL-17A, IL-17A/IL-22 and IL-17A/GM-CSF producing T cells were significantly higher in RRMS patients compared to controls (p< 0.05). Percentages of T cells producing IFN-γ were lower in RRMS patients (p< 0.05). Patients in relapse showed higher percentages of CD4+ T cells producing IL-5, GM-CSF and IL-13 (p=0.05) and lower percentages of CD8+ T cells producing IL-2 (p< 0.05) compared to patients not in relapse. We found a positive correlation between percentages of T cells making IL-13 and the EDSS (r=0.5; p< 0.05) in RRMS patients.
Conclusions: Differences in IL-17, IL-22, GM-CSF and IFN-γ production by CSF cells in RRMS vs. control subjects were observed in this study. Notably, we observed a positive correlation between percentage of T cells producing IL-13 and EDSS in RRMS patients.
Disclosure:
AHC was supported in part by the Manny & Rosalyn Rosenthal.
Dr. John L. Trotter MS Center Chair in Neuroimmunology of the Barnes-Jewish Hospital Foundation.
LP was supported by the Harry Weaver Neuroscience Scholar of the National Multiple Sclerosis Society (NMSS, JF 2144A2/1).
CC was supported during the course of this study by a FISM fellowship (2012/B/1) and subsequently by a NMSS fellowship (FG 2010-A1/2).