Contributions
Abstract: P632
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - 25 Biomarkers
Objective: To explorer the relationship between three cerebrospinal fluid (CSF) biomarkers of poor prognosis (IgM Oligoclonal Bands -OCMB-; Light Neurofilaments -NFl-; and of Chitinase 3-like 1 -CHI3L1-) and the clinic phenotype of multiple sclerosis.
Methods: The CSF of 153 MS patients (94 with relapsing-remitting MS -61.4%; 36 with secondary progressive MS -23.5%; and 23 with primary progressive MS -15.0%-) has been studied, in 40 of them the CSF was obtained during a relapse. OCMB was analyzed by isoelectric focusing and immunodetection in membranes preincubated with the lipids of interest. Determination of levels of light chain Neurofilaments (NF-L) and of Chitinase 3-like 1 (CHI3L1) were quantified by enzyme immunoassay and are detected by absorbance. The disability was measured by the EDSS and the Multiple Sclerosis Severety Score (MSSS).
Results: OCMB were present in 72 patients (47.1); the EDSS was no different between patients with or without OCMB, but the MSSS was higher in patients with OCMB (4.5 vs. 5.6, p=0.01). The mean CHI31L was 168 ng/ml, significant higher in patients with OCMB (p=0.003); the mean NFl concentration was 991 pg/ml, with no no differences in patient with or without OCMB. CHI31L correlated with the EDSS and the MSSS (p=0.013, and p< 0.000, respectively); but NFl shown only a modest correlation only with the MSSS (p=0.04). The correlation between CHI31L and NFl was high (Spearman correlation 0.439). CHI31L was significant higher in progressive MS (p=0.002, Kruskal-Wallis), and independent of relapses; NFl was higher in the CSF obtained during a relapse (1765 pg/ml, p=0.003).
Conclusions: A relationship between the presence of OCMB and CHI31L has been proved, as the expression of a subset of more aggressive patients, according to the high MSSS observed and the evolution to the progressive MSS. In respect to NFl, our work underlying the relationship between the acute axonal destruction and the elevation of NFl, and demonstrate a correlation between the axonal destruction and the microglial activated biomarker CHI3L1.
Teake together our results reinforced the role of the innate immune system in the developing progressive MS.
Disclosure:
Jessica Castillo, Laura Cubas, Sara Gil-Perotin, have not to disclose
Carmen Alcala have received a grant from Genzyme.
Francisco Pérez-Miralles have received grants form Roche, Novartis, Almirall and Genzyme
Bpnaventura Casanova have received grants from Roche, Novartis, TEVA, Genzyme, Bayer-Schering and Almirall.
Abstract: P632
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - 25 Biomarkers
Objective: To explorer the relationship between three cerebrospinal fluid (CSF) biomarkers of poor prognosis (IgM Oligoclonal Bands -OCMB-; Light Neurofilaments -NFl-; and of Chitinase 3-like 1 -CHI3L1-) and the clinic phenotype of multiple sclerosis.
Methods: The CSF of 153 MS patients (94 with relapsing-remitting MS -61.4%; 36 with secondary progressive MS -23.5%; and 23 with primary progressive MS -15.0%-) has been studied, in 40 of them the CSF was obtained during a relapse. OCMB was analyzed by isoelectric focusing and immunodetection in membranes preincubated with the lipids of interest. Determination of levels of light chain Neurofilaments (NF-L) and of Chitinase 3-like 1 (CHI3L1) were quantified by enzyme immunoassay and are detected by absorbance. The disability was measured by the EDSS and the Multiple Sclerosis Severety Score (MSSS).
Results: OCMB were present in 72 patients (47.1); the EDSS was no different between patients with or without OCMB, but the MSSS was higher in patients with OCMB (4.5 vs. 5.6, p=0.01). The mean CHI31L was 168 ng/ml, significant higher in patients with OCMB (p=0.003); the mean NFl concentration was 991 pg/ml, with no no differences in patient with or without OCMB. CHI31L correlated with the EDSS and the MSSS (p=0.013, and p< 0.000, respectively); but NFl shown only a modest correlation only with the MSSS (p=0.04). The correlation between CHI31L and NFl was high (Spearman correlation 0.439). CHI31L was significant higher in progressive MS (p=0.002, Kruskal-Wallis), and independent of relapses; NFl was higher in the CSF obtained during a relapse (1765 pg/ml, p=0.003).
Conclusions: A relationship between the presence of OCMB and CHI31L has been proved, as the expression of a subset of more aggressive patients, according to the high MSSS observed and the evolution to the progressive MSS. In respect to NFl, our work underlying the relationship between the acute axonal destruction and the elevation of NFl, and demonstrate a correlation between the axonal destruction and the microglial activated biomarker CHI3L1.
Teake together our results reinforced the role of the innate immune system in the developing progressive MS.
Disclosure:
Jessica Castillo, Laura Cubas, Sara Gil-Perotin, have not to disclose
Carmen Alcala have received a grant from Genzyme.
Francisco Pérez-Miralles have received grants form Roche, Novartis, Almirall and Genzyme
Bpnaventura Casanova have received grants from Roche, Novartis, TEVA, Genzyme, Bayer-Schering and Almirall.