Contributions
Abstract: EP1498
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Introduction: NeuroBehcet disease (NBD) is the most frequent vasculitis in our country. In presence of inaugural neurological forms, CSF study is systematic because of diagnosis difficulties.
Objectives: To describe the immunoelectrophoretic and cytokine profile in NBD
Patients: Criteria of selection: NBD according to the International consensus recommendation (ICR) with definite and probable forms including isolated neurological syndrome suggestive of NBD. Subjects were consecutive patients who referred to Neurological department of Charles Nicolle hospital since 2013. Blood and CSF samples are taken from all patients. Cell counting, protein analysis, immunoelectrophoretic profile with Ig G Index was performed. Cytokines levels were evaluated by PCR.
Result: From 26 NBD recruited 17 had an inaugural neurological form of which 15/17 had a parenchymal involvement. The IgG index was increased in only 2 patients (8%). At the Immunoelectrophoresis, the profile was type 1 in 22 patients (81%), type 2 in 1 patient (3.7%) and type 3 and 4 in 4 patients (11%). The study of lymphocyte populations showed an increase of IL-17, interferon gamma and IL10 levels in 88% of patients.
Discussion: The profile data in CSF of NBD patients in our study are in line with those found in the literature. Given the limited data on CSF levels of cytokines in patients with NBD, our results showed that cytokines make a pivotal role in pathogenesis of NB, as evidenced by the conjoining increase of pro and anti-inflammatory cytokines in our patients.
Conclusion: Activation of self-reactive T cells with involvement of cytokines and proinflammatory transcription factors is currently proven in NBD. Implication of B cells can be also proved by the presence of oligo-clonal bands.
Disclosure: Nothing to disclose
Abstract: EP1498
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Introduction: NeuroBehcet disease (NBD) is the most frequent vasculitis in our country. In presence of inaugural neurological forms, CSF study is systematic because of diagnosis difficulties.
Objectives: To describe the immunoelectrophoretic and cytokine profile in NBD
Patients: Criteria of selection: NBD according to the International consensus recommendation (ICR) with definite and probable forms including isolated neurological syndrome suggestive of NBD. Subjects were consecutive patients who referred to Neurological department of Charles Nicolle hospital since 2013. Blood and CSF samples are taken from all patients. Cell counting, protein analysis, immunoelectrophoretic profile with Ig G Index was performed. Cytokines levels were evaluated by PCR.
Result: From 26 NBD recruited 17 had an inaugural neurological form of which 15/17 had a parenchymal involvement. The IgG index was increased in only 2 patients (8%). At the Immunoelectrophoresis, the profile was type 1 in 22 patients (81%), type 2 in 1 patient (3.7%) and type 3 and 4 in 4 patients (11%). The study of lymphocyte populations showed an increase of IL-17, interferon gamma and IL10 levels in 88% of patients.
Discussion: The profile data in CSF of NBD patients in our study are in line with those found in the literature. Given the limited data on CSF levels of cytokines in patients with NBD, our results showed that cytokines make a pivotal role in pathogenesis of NB, as evidenced by the conjoining increase of pro and anti-inflammatory cytokines in our patients.
Conclusion: Activation of self-reactive T cells with involvement of cytokines and proinflammatory transcription factors is currently proven in NBD. Implication of B cells can be also proved by the presence of oligo-clonal bands.
Disclosure: Nothing to disclose