Contributions
Abstract: EP1486
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Background: There is a growing interest on modulation of biophysical properties of immune cells. Cell deformation and mechanical phenotyping is of special interest as cytoskletal alterations are supposed to be intimately connected with important cell function. The real-time deformability cytometry (RT-DC) is a novel method to characterize mechanical properties of cells. Previous reports presented how RT-DC can distinguish cell-cycle phases, stem cell differentiation and cell populations in whole blood by their mechanical fingerprints. It is discussed, that physiological and pathological changes could be reflected by mechanical phenotyping. Only few data are available regard mechanical phenotyping in immune cells and no studies are available in MS.
Methods: With RT-DC large populations of cells can be analyzed regard cytoskeletal alterations. Cells are flowed through a microfluid channel constriction and deformed by shear stress and pressure gradients. First, deformation of different immune cell subtypes was evaluated. By in vitro analyses changes in deformation of different lymphocyte subtypes and antigen-presenting cells upon maturation and activation were investigated. Additionally, differences in cell mechanical properties between MS patients and healthy controls were evaluated. Impact of monoclonal-antibody therapies were assessed.
Results: There are distinct differences in deformation between lymphocytes and monocytes/granulocytes in investigated blood samples. Upon stimulation and maturation T cell subtypes presented increased deformation compared to immature state. Additional analyses over time as defined by time kinetic measurements presented selective changes in degree of deformation with development and selection of different subgroups defined by various degrees in deformation. There were distinct changes in deformation of peripheral immune cell subtypes directly after monocloncal-antibody application including natalizumab, alemtuzumab and ocrelizumab treatment in MS patients. Impact on deformation differed between acute versus long-term effects during monoclonal antibody therapy.
Conclusion: Here we present distinct patterns of cell mechanical characteristics of different peripheral immune cell subtypes and its modulation during maturation and activation. First data indicate selective impact on cell mechanical properties during treatment. Further studies will elucidate how cell mechanical phenotyping can act as biomarker in clinical practice.
Disclosure:
K. Thomas received personal compensation for from Novartis, Biogen Idec and Roche for consulting service. Ziemssen received personal compensation from Biogen Idec, Bayer, Novartis, Sanofi, Teva, and Synthon for consulting services.
Ziemssen received additional financial support for research activities from Bayer, Biogen Idec, Novartis, Teva, and Sanofi Aventis.
P. Shalchi Amirkhiz has nothing to disclose.
Abstract: EP1486
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Background: There is a growing interest on modulation of biophysical properties of immune cells. Cell deformation and mechanical phenotyping is of special interest as cytoskletal alterations are supposed to be intimately connected with important cell function. The real-time deformability cytometry (RT-DC) is a novel method to characterize mechanical properties of cells. Previous reports presented how RT-DC can distinguish cell-cycle phases, stem cell differentiation and cell populations in whole blood by their mechanical fingerprints. It is discussed, that physiological and pathological changes could be reflected by mechanical phenotyping. Only few data are available regard mechanical phenotyping in immune cells and no studies are available in MS.
Methods: With RT-DC large populations of cells can be analyzed regard cytoskeletal alterations. Cells are flowed through a microfluid channel constriction and deformed by shear stress and pressure gradients. First, deformation of different immune cell subtypes was evaluated. By in vitro analyses changes in deformation of different lymphocyte subtypes and antigen-presenting cells upon maturation and activation were investigated. Additionally, differences in cell mechanical properties between MS patients and healthy controls were evaluated. Impact of monoclonal-antibody therapies were assessed.
Results: There are distinct differences in deformation between lymphocytes and monocytes/granulocytes in investigated blood samples. Upon stimulation and maturation T cell subtypes presented increased deformation compared to immature state. Additional analyses over time as defined by time kinetic measurements presented selective changes in degree of deformation with development and selection of different subgroups defined by various degrees in deformation. There were distinct changes in deformation of peripheral immune cell subtypes directly after monocloncal-antibody application including natalizumab, alemtuzumab and ocrelizumab treatment in MS patients. Impact on deformation differed between acute versus long-term effects during monoclonal antibody therapy.
Conclusion: Here we present distinct patterns of cell mechanical characteristics of different peripheral immune cell subtypes and its modulation during maturation and activation. First data indicate selective impact on cell mechanical properties during treatment. Further studies will elucidate how cell mechanical phenotyping can act as biomarker in clinical practice.
Disclosure:
K. Thomas received personal compensation for from Novartis, Biogen Idec and Roche for consulting service. Ziemssen received personal compensation from Biogen Idec, Bayer, Novartis, Sanofi, Teva, and Synthon for consulting services.
Ziemssen received additional financial support for research activities from Bayer, Biogen Idec, Novartis, Teva, and Sanofi Aventis.
P. Shalchi Amirkhiz has nothing to disclose.