Contributions
Abstract: EP1485
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Background: Optic neuromyelitis (NMO) is an autoimmune, demyelinating, and neurodegenerative disease that primarily affects the optic nerve and spinal cord. AQP4-IgG (IgG-NMO), a specific autoantibody against aquaporin 4 channel is expressed in certain areas of the brain and some particular cells, such as astrocytes, has been linked to the etiopathogenesis of the disease. Our aim was to evaluate in vitro the effect of this immunoglobulin on the proliferation and differentiation capacity of neural progenitor cells in culture to elucidate the possible role of the antibody in the disease.
Methods: Serum samples from six patients diagnosed with NMO and seropositive for IgG-NMO and four healthy controls were used Neurospheres were extracted from young BALB/c mice and cultured in complete medium (DMEM supplemented with hormone mix, trophic factors and 5% FBS), at passage 5 the neurospheres were divided into three groups and the culture media were supplemented with: 2% HC serum; 2% of patients serums with NMO and sham which only contained the culture medium; after 10 days of differentiation the neurospheres were fixed for later analysis by immunohistochemistry for the identification of Tuj1, GFAP and OLIG2.
Results: In the neurosphere cultures, alterations in the differentiation were observed, showing a lower rate of OLIG2 cells (Sham: 20,22 ± 3,2; HC: 21,6 ± 2.4; NMO: 8,93 ± 3,7; P < 0.05). Higher number of GFAP positive cells in patients with NMO compared to sham and HC (Sham: 64,04 ± 3,2; HC: 60,9 ± 4,5; NMO: 80,82 ± 2, 5; P < 0.05) were observed. In the differentiation to the neuronal strain only Tuj1 decrease was found (Sham: 15,74 ± 2,6; HC: 17,5 ± 1.5; NMO: 10,82 ± 3,3; P < 0.05).
Conclusions: The serum affects the proliferation and differentiation of the neurospheres in the different cell lineages, mainly compromising the number of neuronal cells and oligodendroglia.
Disclosure:
Yolanda García Ávila: nothing to disclose
Lucía Gallego Villarejo: nothing to disclose
Vanesa Pytel: nothing to disclose
María Soledad Benito Martín: nothing to disclose
Lidia Moreno Jiménez: nothing to disclose
Laura Torre Fuentes: nothing to disclose
Álvaro Gómez Graña: nothing to disclose
Jordi Matías-Guiu Antem: nothing to disclose
Inés González Suárez: nothing to disclose
C. Oreja-Guevara: nothing to disclose
Ulises Gómez Pinedo: nothing to disclose
Jorge Matías-Guiu Guía: nothing to disclose
Abstract: EP1485
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - 15 Immunology
Background: Optic neuromyelitis (NMO) is an autoimmune, demyelinating, and neurodegenerative disease that primarily affects the optic nerve and spinal cord. AQP4-IgG (IgG-NMO), a specific autoantibody against aquaporin 4 channel is expressed in certain areas of the brain and some particular cells, such as astrocytes, has been linked to the etiopathogenesis of the disease. Our aim was to evaluate in vitro the effect of this immunoglobulin on the proliferation and differentiation capacity of neural progenitor cells in culture to elucidate the possible role of the antibody in the disease.
Methods: Serum samples from six patients diagnosed with NMO and seropositive for IgG-NMO and four healthy controls were used Neurospheres were extracted from young BALB/c mice and cultured in complete medium (DMEM supplemented with hormone mix, trophic factors and 5% FBS), at passage 5 the neurospheres were divided into three groups and the culture media were supplemented with: 2% HC serum; 2% of patients serums with NMO and sham which only contained the culture medium; after 10 days of differentiation the neurospheres were fixed for later analysis by immunohistochemistry for the identification of Tuj1, GFAP and OLIG2.
Results: In the neurosphere cultures, alterations in the differentiation were observed, showing a lower rate of OLIG2 cells (Sham: 20,22 ± 3,2; HC: 21,6 ± 2.4; NMO: 8,93 ± 3,7; P < 0.05). Higher number of GFAP positive cells in patients with NMO compared to sham and HC (Sham: 64,04 ± 3,2; HC: 60,9 ± 4,5; NMO: 80,82 ± 2, 5; P < 0.05) were observed. In the differentiation to the neuronal strain only Tuj1 decrease was found (Sham: 15,74 ± 2,6; HC: 17,5 ± 1.5; NMO: 10,82 ± 3,3; P < 0.05).
Conclusions: The serum affects the proliferation and differentiation of the neurospheres in the different cell lineages, mainly compromising the number of neuronal cells and oligodendroglia.
Disclosure:
Yolanda García Ávila: nothing to disclose
Lucía Gallego Villarejo: nothing to disclose
Vanesa Pytel: nothing to disclose
María Soledad Benito Martín: nothing to disclose
Lidia Moreno Jiménez: nothing to disclose
Laura Torre Fuentes: nothing to disclose
Álvaro Gómez Graña: nothing to disclose
Jordi Matías-Guiu Antem: nothing to disclose
Inés González Suárez: nothing to disclose
C. Oreja-Guevara: nothing to disclose
Ulises Gómez Pinedo: nothing to disclose
Jorge Matías-Guiu Guía: nothing to disclose