Contributions
Abstract: P595
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Biomarkers
Background: Clinical and experimental evidence suggests that endothelin-1 (ET-1) plays a role in cardiac and vascular disease and inhibits remyelination. Patients with multiple sclerosis (MS) show global cerebral hypoperfusion. The widespread decrease in perfusion in normal-appearing white matter and grey matter in MS seems to be secondary to increased blood concentrations of ET-1.
Objectives: To evaluate ET-1 in MS patients vs matched healthy subjects (HS) in the context of a larger study on the association among MS, amyotrophic lateral sclerosis (ALS), and vascular changes at molecular, genetic, anatomic and functional level.
Methods: ET-1 serum levels were measured in MS patients, ALS patients and HS recruited over 3 years, with a commercially available ELISA kit, in blinded fashion by a laboratory assistant (detection range 0.39-25 pg/mL; R&D Systems). Clinical and demographic characteristics of patients and HS were recorded.
Results: ET-1 serum levels were analysed in 274 MS patients (162 females, 112 males), 284 HS (168 females, 116 males) and in 35 ALS (16 females, 19 males). ET-1 median levels were significantly higher in MS compared to HS (1.73 vs 1.48 pg/mL) and were nonsignificant in ALS subjects compared to 37 matched HS. In MS patients ET-1 levels were positively related to age, disease duration (DD) and EDSS. In MS women ET-1 levels positively correlated with age (p= 0.014) and DD (p= 0.024), while in MS males they tended to be related to EDSS (p=0.058). No significant relationship was found between age and ET-1 in female HS. The mean values for age and DD in MS women vs men were nonsignificant.
Conclusions: We confirm that serum ET-1 levels are significantly increased in MS patients. Furthermore, ET-1 levels are related to age, DD, and EDSS in MS patients in a different way between genders. These findings suggest a role for ET-1 in MS disease, and could also contribute to explain the different incidence and disease course of MS in men and women.
Study Support: Research and University Ministery.
Disclosure:
Criscuolo Chiara: nothing to disclose
Lanzillo Roberta: nothing to disclose
Cianflone Alessandra: nothing to disclose
Liuzzi Raffaele: nothing to disclose
Di Taranto Maria Donata: nothing to disclose
Incoronato Mariarosaria: nothing to disclose
Moccia Marcello: nothing to disclose
Palladino Raffele: nothing to disclose
Caporale Oreste: nothing to disclose
Triassi Maria: nothing to disclose
Salvatore Marco: nothing to disclose
Brescia Morra Vincenzo: nothing to disclose
Abstract: P595
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Biomarkers
Background: Clinical and experimental evidence suggests that endothelin-1 (ET-1) plays a role in cardiac and vascular disease and inhibits remyelination. Patients with multiple sclerosis (MS) show global cerebral hypoperfusion. The widespread decrease in perfusion in normal-appearing white matter and grey matter in MS seems to be secondary to increased blood concentrations of ET-1.
Objectives: To evaluate ET-1 in MS patients vs matched healthy subjects (HS) in the context of a larger study on the association among MS, amyotrophic lateral sclerosis (ALS), and vascular changes at molecular, genetic, anatomic and functional level.
Methods: ET-1 serum levels were measured in MS patients, ALS patients and HS recruited over 3 years, with a commercially available ELISA kit, in blinded fashion by a laboratory assistant (detection range 0.39-25 pg/mL; R&D Systems). Clinical and demographic characteristics of patients and HS were recorded.
Results: ET-1 serum levels were analysed in 274 MS patients (162 females, 112 males), 284 HS (168 females, 116 males) and in 35 ALS (16 females, 19 males). ET-1 median levels were significantly higher in MS compared to HS (1.73 vs 1.48 pg/mL) and were nonsignificant in ALS subjects compared to 37 matched HS. In MS patients ET-1 levels were positively related to age, disease duration (DD) and EDSS. In MS women ET-1 levels positively correlated with age (p= 0.014) and DD (p= 0.024), while in MS males they tended to be related to EDSS (p=0.058). No significant relationship was found between age and ET-1 in female HS. The mean values for age and DD in MS women vs men were nonsignificant.
Conclusions: We confirm that serum ET-1 levels are significantly increased in MS patients. Furthermore, ET-1 levels are related to age, DD, and EDSS in MS patients in a different way between genders. These findings suggest a role for ET-1 in MS disease, and could also contribute to explain the different incidence and disease course of MS in men and women.
Study Support: Research and University Ministery.
Disclosure:
Criscuolo Chiara: nothing to disclose
Lanzillo Roberta: nothing to disclose
Cianflone Alessandra: nothing to disclose
Liuzzi Raffaele: nothing to disclose
Di Taranto Maria Donata: nothing to disclose
Incoronato Mariarosaria: nothing to disclose
Moccia Marcello: nothing to disclose
Palladino Raffele: nothing to disclose
Caporale Oreste: nothing to disclose
Triassi Maria: nothing to disclose
Salvatore Marco: nothing to disclose
Brescia Morra Vincenzo: nothing to disclose