Contributions
Abstract: P514
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Imaging
Background: Diffusion tensor imaging (DTI) has been applied in clinically isolated syndrome (CIS), and demonstrated the presence of pathological abnormalities in the normal-appearing white matter (NAWM). However, DTI indices have a low pathological specificity. Neurite Orientation Dispersion and Density Imaging (NODDI) is a new diffusion weighted multi-compartment model, which reflects neuronal morphology, like the density and the dispersion of the neurites (axons and dendrites). Preliminary applications to patients with relapsing-remitting multiple sclerosis have shown abnormal NODDI parameters in the NAWM and grey matter (GM).
Objective: The aims of this study were to: (i) compare the NODDI parameters between CIS patients and healthy controls (HCs) (ii) determine in CIS patients the relationship between NODDI measures and clinical scores.
Methods: We recruited: 21 CIS subjects within 3 months from symptom onset (10 females), mean age 34.25 years (SD8.57) and 12 HC (8 females), mean age 33.73 years (SD7.18). The brain MRI protocol performed at 3T included T2-images, volumetric images and NODDI. 14/20 patients had asymptomatic T2 WM lesions. Brain tissue segmentation was performed to obtain white matter (WM) and GM tissue volumes (after lesion filling). Using the NODDI matlab toolbox we obtained: orientation dispersion index (ODI), neurite density index (NDI) and isotropic volume fraction (isoVF); they were calculated in WM, GM and T2 lesions. The Expanded Disability Status Scale (EDSS), MS Functional Composite score, (MSFC) and the Brief Cognitive Assessment for MS (BICAMS) were scored in the patient group. Linear regression models were used to compare the differences in MRI measures between groups adjusting for age, gender and brain volume fractions.
Results: Patients had a median EDSS of 1.0 (range 0 - 2.5) and were cognitively intact. Patients showed a higher orientation dispersion index (ODI) in NAWM than WM of HCs (0.26 ±0.1 vs 0.25±0.05 p< 0.023). The other NODDI parameters did not differ between CIS and HCs. The NAWM ODI did not correlate with clinical scores.
Conclusion: Our results suggest a reduced alignment of axons that become more dispersed and less oriented when compared to those of the normal WM. These results now need to be confirmed in a larger sample (recruitment is ongoing).
Disclosure:
S. Collorone Meeting expenses AAN conference 2016 paid by Novartis.
N. Cawley Nothing to disclose
F. Prados Nothing to disclose
C. Tur has received an ECTRIMS post-doctoral research fellowship in 2015. She has also received honoraria and support for travelling from Bayer-Schering, Teva, Merck-Serono and Serono Foundation, Biogen, Sanofi-Aventis, Novartis, and Ismar Healthcare
F. Grussu is funded by the H2020-EU.3.1 CDS-QUAMRI grant (ref.: 634541) and received support from the UCL Grand Challenge Studentships scheme.
B. Kanber Nothing to disclose
S. Ourselin Nothing to disclose
CAM Gandini Wheeler-Kingshott is on the editorial board of Functional Neurology and receives research grants (PI and co-applicant) from ISRT, EPSRC, Wings for Life, UK MS Society, Horizon2020, Biogen and Novartis.
D.H Miller received grants from UCL/UCLH Biomedical Research Centre, during the conduct of the study; has received grants from Biogen, GlaxoSmithKline, the National Institute for Health Research, Novartis, and Apitope; has board membership with Biogen Idec, GlaxoSmithKline, Bayer Schering Pharma, and Mitsubishi Pharma Europe; has been a consultant for Merck and Chugai; and has received personal fees from McAlpine"s Multiple Sclerosis, 4th edition.
A. Thompson Received honorarium from SAGE Publishers as Editor-in-Chief for Multiple Sclerosis Journal. Free subscription from Elsevier as member of Editorial Board for The Lancet Neurology . Received personal compensation for consulting, serving on a scientific advisory board and speaking from Biogen, MedDay, Novartis and Teva.
A.Toosy has received speaker honoraria from Biomedia, Sereno Symposia International Foundation, Bayer and meeting expenses from Biogen Idec.
O. Ciccarelli is an Associate Editor of Neurology and serves as a consultant for GE Healthcare, Novartis, Roche, Biogen, Genzyme and Teva.
Abstract: P514
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Imaging
Background: Diffusion tensor imaging (DTI) has been applied in clinically isolated syndrome (CIS), and demonstrated the presence of pathological abnormalities in the normal-appearing white matter (NAWM). However, DTI indices have a low pathological specificity. Neurite Orientation Dispersion and Density Imaging (NODDI) is a new diffusion weighted multi-compartment model, which reflects neuronal morphology, like the density and the dispersion of the neurites (axons and dendrites). Preliminary applications to patients with relapsing-remitting multiple sclerosis have shown abnormal NODDI parameters in the NAWM and grey matter (GM).
Objective: The aims of this study were to: (i) compare the NODDI parameters between CIS patients and healthy controls (HCs) (ii) determine in CIS patients the relationship between NODDI measures and clinical scores.
Methods: We recruited: 21 CIS subjects within 3 months from symptom onset (10 females), mean age 34.25 years (SD8.57) and 12 HC (8 females), mean age 33.73 years (SD7.18). The brain MRI protocol performed at 3T included T2-images, volumetric images and NODDI. 14/20 patients had asymptomatic T2 WM lesions. Brain tissue segmentation was performed to obtain white matter (WM) and GM tissue volumes (after lesion filling). Using the NODDI matlab toolbox we obtained: orientation dispersion index (ODI), neurite density index (NDI) and isotropic volume fraction (isoVF); they were calculated in WM, GM and T2 lesions. The Expanded Disability Status Scale (EDSS), MS Functional Composite score, (MSFC) and the Brief Cognitive Assessment for MS (BICAMS) were scored in the patient group. Linear regression models were used to compare the differences in MRI measures between groups adjusting for age, gender and brain volume fractions.
Results: Patients had a median EDSS of 1.0 (range 0 - 2.5) and were cognitively intact. Patients showed a higher orientation dispersion index (ODI) in NAWM than WM of HCs (0.26 ±0.1 vs 0.25±0.05 p< 0.023). The other NODDI parameters did not differ between CIS and HCs. The NAWM ODI did not correlate with clinical scores.
Conclusion: Our results suggest a reduced alignment of axons that become more dispersed and less oriented when compared to those of the normal WM. These results now need to be confirmed in a larger sample (recruitment is ongoing).
Disclosure:
S. Collorone Meeting expenses AAN conference 2016 paid by Novartis.
N. Cawley Nothing to disclose
F. Prados Nothing to disclose
C. Tur has received an ECTRIMS post-doctoral research fellowship in 2015. She has also received honoraria and support for travelling from Bayer-Schering, Teva, Merck-Serono and Serono Foundation, Biogen, Sanofi-Aventis, Novartis, and Ismar Healthcare
F. Grussu is funded by the H2020-EU.3.1 CDS-QUAMRI grant (ref.: 634541) and received support from the UCL Grand Challenge Studentships scheme.
B. Kanber Nothing to disclose
S. Ourselin Nothing to disclose
CAM Gandini Wheeler-Kingshott is on the editorial board of Functional Neurology and receives research grants (PI and co-applicant) from ISRT, EPSRC, Wings for Life, UK MS Society, Horizon2020, Biogen and Novartis.
D.H Miller received grants from UCL/UCLH Biomedical Research Centre, during the conduct of the study; has received grants from Biogen, GlaxoSmithKline, the National Institute for Health Research, Novartis, and Apitope; has board membership with Biogen Idec, GlaxoSmithKline, Bayer Schering Pharma, and Mitsubishi Pharma Europe; has been a consultant for Merck and Chugai; and has received personal fees from McAlpine"s Multiple Sclerosis, 4th edition.
A. Thompson Received honorarium from SAGE Publishers as Editor-in-Chief for Multiple Sclerosis Journal. Free subscription from Elsevier as member of Editorial Board for The Lancet Neurology . Received personal compensation for consulting, serving on a scientific advisory board and speaking from Biogen, MedDay, Novartis and Teva.
A.Toosy has received speaker honoraria from Biomedia, Sereno Symposia International Foundation, Bayer and meeting expenses from Biogen Idec.
O. Ciccarelli is an Associate Editor of Neurology and serves as a consultant for GE Healthcare, Novartis, Roche, Biogen, Genzyme and Teva.