Contributions
Abstract: P456
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Neurobiology
Background: Myeloid cells act as “double edged sword” in the pathogenesis of MS. They play a neuro-destructive role by secreting ROS and free or microvesicles (MVs)-embedded proinflammatory cytokines (classically activated microglia) or neuro-protective functions through the production of anti-inflammatory cytokines and neurotrophic factors (alternatively activated microglia). Recent data suggests that oxidative stress may trigger the release of MVs and, in turn, MVs may generate free radicals by activation of the oxidative stress machinery.
Objective: Preliminary investigation of the relationship between oxidative stress markers and MVs release by myeloid cells in MS patients.
Design and methods: We measured Glutathione levels (GSH, GSSG, GSH/GSSG ratio), slow and fast plasma antioxidant power (PAO), radical oxidative species (ROS), malondialdheide (MDA), Anti-oxidized Low density lipoprotein antibodies (Anti-OxLDL), Coenzyme Q10 (Coq10) levels as well as PBMC derived MVs in peripheral blood of age-and-sex matched Health controls (HC) and patients with relapsing remitting and progressive forms of MS.
Results: Using K-means clustering alghoritms, two cluster patterns of oxidative stress measures were detected : cluster A with prevailing anti-oxidative markers and cluster B with more pro-oxidative stress markers. No differences between HC and MS patients in cluster patterns of oxidative stress were observed. Female patients produced much more MVs than males regardless of the oxidative status. Young patients with high oxidative stress released statistically significant more MVs than young patients with low oxidative stress, while for older patients the relationship is reversed. According to covariance analysis, upon ATP stimulation, patients with prevailing anti-oxidative markers have significantly higher levels of MVs than patients with pro-oxidative stress markers (p 0.01).
Conclusion: A dual relationship between myeloid MVs and oxidative stress markers has been found by these preliminary results, with a positive relationship between the two variable in young patients and the reverse in older patients. In either cases, oxidative stress reduces MVs release after ATP stimulation
Disclosure:
M Gironi, G Dalla Costa, A Finardi, V Martinelli, G Comi and R Furlan
have non potential conflict of interest and didn´t receive any special grant for this study
Abstract: P456
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Neurobiology
Background: Myeloid cells act as “double edged sword” in the pathogenesis of MS. They play a neuro-destructive role by secreting ROS and free or microvesicles (MVs)-embedded proinflammatory cytokines (classically activated microglia) or neuro-protective functions through the production of anti-inflammatory cytokines and neurotrophic factors (alternatively activated microglia). Recent data suggests that oxidative stress may trigger the release of MVs and, in turn, MVs may generate free radicals by activation of the oxidative stress machinery.
Objective: Preliminary investigation of the relationship between oxidative stress markers and MVs release by myeloid cells in MS patients.
Design and methods: We measured Glutathione levels (GSH, GSSG, GSH/GSSG ratio), slow and fast plasma antioxidant power (PAO), radical oxidative species (ROS), malondialdheide (MDA), Anti-oxidized Low density lipoprotein antibodies (Anti-OxLDL), Coenzyme Q10 (Coq10) levels as well as PBMC derived MVs in peripheral blood of age-and-sex matched Health controls (HC) and patients with relapsing remitting and progressive forms of MS.
Results: Using K-means clustering alghoritms, two cluster patterns of oxidative stress measures were detected : cluster A with prevailing anti-oxidative markers and cluster B with more pro-oxidative stress markers. No differences between HC and MS patients in cluster patterns of oxidative stress were observed. Female patients produced much more MVs than males regardless of the oxidative status. Young patients with high oxidative stress released statistically significant more MVs than young patients with low oxidative stress, while for older patients the relationship is reversed. According to covariance analysis, upon ATP stimulation, patients with prevailing anti-oxidative markers have significantly higher levels of MVs than patients with pro-oxidative stress markers (p 0.01).
Conclusion: A dual relationship between myeloid MVs and oxidative stress markers has been found by these preliminary results, with a positive relationship between the two variable in young patients and the reverse in older patients. In either cases, oxidative stress reduces MVs release after ATP stimulation
Disclosure:
M Gironi, G Dalla Costa, A Finardi, V Martinelli, G Comi and R Furlan
have non potential conflict of interest and didn´t receive any special grant for this study