Contributions
Abstract: P444
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - MS and infections
Objective: Mycobacterium avium subsp. paratuberculosis (MAP) is an intracellular pathogen which can infect both animals and humans. MAP has recently been associated with multiple sclerosis (MS) in patients from Italy. We aimed to study the serology of MAP in a population characterized by a different ethnic and genetic background such as Japan, looking at several markers of MAP presence.
Methods: A total of 50 MS patients free-therapy compared to 50 sex-and age-matched healthy controls, all from Japan, were tested by indirect ELISA for the presence of antibodies (Abs) toward the following epitopes: MAP_0106c121-132 and the human homologues MBP85-98, MAP_402718-32 and humanhomologues IRFF5424-432, and MAP_2694295-303.
Results: Amongst all the peptides, only Abs against MAP_2694295-303 epitope, which share homology with human gamma delta T cell receptor, were significantly recognize in MS patients (30%) compared to controls (2%) (p = 0.0004).
Conclusions: These findings support the view that MAP could act as risk factor or a triggering agent of MS also in some Japanese patients with a genetic susceptibility to the mycobacterium. On the other side, the lack of humoral response against several epitopes suggested that the association of a specific environmental pathogen with causation/progression of a disease such as MS can be population dependent, and the immune response directed against multiple epitopes depends on different genetic and non-genetic factors.
Disclosure:
Cossu Davide: nothing to disclose
Yokoyama Kazumasa: nothing to disclose
Hoshino Yasunobu: nothing to disclose
Tomizawa Yuji: nothing to disclose
Hattori Nobutaka: nothing to disclose
Abstract: P444
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - MS and infections
Objective: Mycobacterium avium subsp. paratuberculosis (MAP) is an intracellular pathogen which can infect both animals and humans. MAP has recently been associated with multiple sclerosis (MS) in patients from Italy. We aimed to study the serology of MAP in a population characterized by a different ethnic and genetic background such as Japan, looking at several markers of MAP presence.
Methods: A total of 50 MS patients free-therapy compared to 50 sex-and age-matched healthy controls, all from Japan, were tested by indirect ELISA for the presence of antibodies (Abs) toward the following epitopes: MAP_0106c121-132 and the human homologues MBP85-98, MAP_402718-32 and humanhomologues IRFF5424-432, and MAP_2694295-303.
Results: Amongst all the peptides, only Abs against MAP_2694295-303 epitope, which share homology with human gamma delta T cell receptor, were significantly recognize in MS patients (30%) compared to controls (2%) (p = 0.0004).
Conclusions: These findings support the view that MAP could act as risk factor or a triggering agent of MS also in some Japanese patients with a genetic susceptibility to the mycobacterium. On the other side, the lack of humoral response against several epitopes suggested that the association of a specific environmental pathogen with causation/progression of a disease such as MS can be population dependent, and the immune response directed against multiple epitopes depends on different genetic and non-genetic factors.
Disclosure:
Cossu Davide: nothing to disclose
Yokoyama Kazumasa: nothing to disclose
Hoshino Yasunobu: nothing to disclose
Tomizawa Yuji: nothing to disclose
Hattori Nobutaka: nothing to disclose