Contributions
Abstract: P429
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Immunology
Objective: Data from animal models, epidemiologic and metabolomics studies suggest a role of the gastrointestinal tract in multiple sclerosis (MS). We wanted to substantiate this assumption by directly investigating the immune cells of the colonic mucosa in treatment-naïve patients with a clinically isolated syndrome (CIS) or early relapsing MS and to assess a possible association with the faecal content in short-chain fatty acids (SCFA).
Methods: We obtained mucosal specimen during ileocolonoscopy from fifteen CIS /MS patients and ten controls. Mucosal immune cells were analyzed by Fluorescence-activated cell sorting (FACS) and gas chromatography-mass spectrometry (GC-MS) measurements of stool samples served to determine SCFA.
Results: The number of total dendritic cells (DC), CD103+ tolerogenic DCs and CD4+CD25+127- regulatory T-cells (Tregs) was significantly reduced in the left-sided colon of CIS/MS compared to controls while we found no differences on the right side. The patients" faecal samples also showed a substantially lower content of SFCA and especially lower levels of butyrate and acetate.
Interpretation: Our findings indicate a disturbed homeostasis of colonic DCs and Tregs in MS patients which could be associated to colonic SCFA depletion. While not implying causality these findings strengthen a role of the gut in MS and warrant further research if modulation of the colonic SCFA profile or the colonic Treg pool can serve to modify the course of MS.
Disclosure:
Dr. Spindelboeck: nothing to disclose
Dr. Moser: nothing to disclose
Dr. Strohmaier: nothing to disclose
Dr. Enzinger: nothing to disclose
Dr. Gattringer: nothing to disclose
Dr. Fuchs: nothing to disclose
Dr. Fazekas: nothing to disclose
Dr. Gorkiewicz: nothing to disclose
Mr. Wurm, Msc: nothing to disclose
Dr. Högenauer: nothing to disclose
Dr. Khalil: nothing to disclose
Abstract: P429
Type: Poster
Abstract Category: Pathology and pathogenesis of MS - Immunology
Objective: Data from animal models, epidemiologic and metabolomics studies suggest a role of the gastrointestinal tract in multiple sclerosis (MS). We wanted to substantiate this assumption by directly investigating the immune cells of the colonic mucosa in treatment-naïve patients with a clinically isolated syndrome (CIS) or early relapsing MS and to assess a possible association with the faecal content in short-chain fatty acids (SCFA).
Methods: We obtained mucosal specimen during ileocolonoscopy from fifteen CIS /MS patients and ten controls. Mucosal immune cells were analyzed by Fluorescence-activated cell sorting (FACS) and gas chromatography-mass spectrometry (GC-MS) measurements of stool samples served to determine SCFA.
Results: The number of total dendritic cells (DC), CD103+ tolerogenic DCs and CD4+CD25+127- regulatory T-cells (Tregs) was significantly reduced in the left-sided colon of CIS/MS compared to controls while we found no differences on the right side. The patients" faecal samples also showed a substantially lower content of SFCA and especially lower levels of butyrate and acetate.
Interpretation: Our findings indicate a disturbed homeostasis of colonic DCs and Tregs in MS patients which could be associated to colonic SCFA depletion. While not implying causality these findings strengthen a role of the gut in MS and warrant further research if modulation of the colonic SCFA profile or the colonic Treg pool can serve to modify the course of MS.
Disclosure:
Dr. Spindelboeck: nothing to disclose
Dr. Moser: nothing to disclose
Dr. Strohmaier: nothing to disclose
Dr. Enzinger: nothing to disclose
Dr. Gattringer: nothing to disclose
Dr. Fuchs: nothing to disclose
Dr. Fazekas: nothing to disclose
Dr. Gorkiewicz: nothing to disclose
Mr. Wurm, Msc: nothing to disclose
Dr. Högenauer: nothing to disclose
Dr. Khalil: nothing to disclose