Contributions
Abstract: P1673
Type: LB Poster
Abstract Category: Late Breaking News
The pathophysiology of multiple sclerosis (MS) is heterogeneous and complex. γδ T cells are found in active and chronic active lesions along with other T cell subsets.
In this context, we aimed to quantify and functional characterize γδT subsets in the peripheral blood (PB) from patients diagnosed with MS, according to the MacDonald criteria2010.
γδ T subsets were defined by the concomitant expression of CD45Ra and CD27 in naive, central memory TCM, effector memory TEM, and terminally differentiated effector memory TEMRA, the expression of CCR5 was studied in each subset. Functional characterization was done by intracellular cytokine staining after in vitro stimulation with PMA/ionomycin, in the presence of Brefeldin A during 4 hours. The expression of cell surface markers and intracellular production of IL-17, TNFα, IFNγ and IL-2 were assessed by flow cytometry (FACSCanto II; BD), and data were analyzed using Infinicyt software (Cytognos). The non-parametric Mann-Whitney U test (average ± standard deviation; median) was used for statistical evaluation.
38 MS patients, 30 of them in remission (26 female, mean age 41±15) and 8 in relapse (5 female, mean age 44±11), and 20 healthy control (HC) (16 female, mean age 50±9) were evaluated.
In MS patients in remission we observed an increase in the frequency of naive (15,1±10,7 vs 5,0±5,8) and a decreased in frequency of CM γδT cells (48,4±22,5 vs 58,1±25,1), when compared with HC.
MS patients in relapse also exhibited an increased frequency of naive γδT cells (22,1±32,1 vs 5,0±5,8) when compared with HC and a decreased of TEMRA (8,2±11,3 vs 21,3±17,1) when compared with MS in remission. The frequency of TEMRA expressing CCR5 is decreased (2,8±2,8 vs 11,2±15,8) when compared with MS in remission. Moreover, an increase of IFN-γ+ γδT cells was observed in MS in relapse (75,1±12,4 vs 57,4±19,2 in MS in remission).
In summary, it seems that γδT subsets could play an important role in the pathophysiology and progression of MS, since a decreased frequency of CCR5+ TEMRA and an increased of IFN-γ+ γδT cells were observed in PB from MS patients in relapse, which could reflect a specific migration to central nervous system. A more comprehensive knowledge of the plasticity of these cells in MS is highly pertinent for future therapeutic interventions.
Disclosure: All: nothing to disclose
Abstract: P1673
Type: LB Poster
Abstract Category: Late Breaking News
The pathophysiology of multiple sclerosis (MS) is heterogeneous and complex. γδ T cells are found in active and chronic active lesions along with other T cell subsets.
In this context, we aimed to quantify and functional characterize γδT subsets in the peripheral blood (PB) from patients diagnosed with MS, according to the MacDonald criteria2010.
γδ T subsets were defined by the concomitant expression of CD45Ra and CD27 in naive, central memory TCM, effector memory TEM, and terminally differentiated effector memory TEMRA, the expression of CCR5 was studied in each subset. Functional characterization was done by intracellular cytokine staining after in vitro stimulation with PMA/ionomycin, in the presence of Brefeldin A during 4 hours. The expression of cell surface markers and intracellular production of IL-17, TNFα, IFNγ and IL-2 were assessed by flow cytometry (FACSCanto II; BD), and data were analyzed using Infinicyt software (Cytognos). The non-parametric Mann-Whitney U test (average ± standard deviation; median) was used for statistical evaluation.
38 MS patients, 30 of them in remission (26 female, mean age 41±15) and 8 in relapse (5 female, mean age 44±11), and 20 healthy control (HC) (16 female, mean age 50±9) were evaluated.
In MS patients in remission we observed an increase in the frequency of naive (15,1±10,7 vs 5,0±5,8) and a decreased in frequency of CM γδT cells (48,4±22,5 vs 58,1±25,1), when compared with HC.
MS patients in relapse also exhibited an increased frequency of naive γδT cells (22,1±32,1 vs 5,0±5,8) when compared with HC and a decreased of TEMRA (8,2±11,3 vs 21,3±17,1) when compared with MS in remission. The frequency of TEMRA expressing CCR5 is decreased (2,8±2,8 vs 11,2±15,8) when compared with MS in remission. Moreover, an increase of IFN-γ+ γδT cells was observed in MS in relapse (75,1±12,4 vs 57,4±19,2 in MS in remission).
In summary, it seems that γδT subsets could play an important role in the pathophysiology and progression of MS, since a decreased frequency of CCR5+ TEMRA and an increased of IFN-γ+ γδT cells were observed in PB from MS patients in relapse, which could reflect a specific migration to central nervous system. A more comprehensive knowledge of the plasticity of these cells in MS is highly pertinent for future therapeutic interventions.
Disclosure: All: nothing to disclose