Contributions
Abstract: P1666
Type: LB Poster
Abstract Category: Late Breaking News
Background and goals: Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS) with an autoimmune reaction that cause distinct clinical courses.The immunopathological differences between relapsing-remitting (RRMS) and primary progressive multiple sclerosis (PPMS) are not fully explored.Neurofilament light polypeptide (NFL) is an intracellular protein supplying cytoskeleton of neurons. Axonal damage is associated with NFL degradation and secretion into the CSF.NFL levels in CSF probably reflects the degree of MS-associated neurodegeneration.Gangliosides constitute an axonal antigen and are also minor constituents of myelin. The synthesis of pathogenic autoantibodies to gangliosides may cause an injury to neuronal tissue and induce a complex local immune process. Interleukin 18 (IL-18) is an pro-inflammatory cytokine which is a costimulatory factor for production of IFN-gamma by T helper 1-type cells.
The aim of the study is to investigate the concentrations of NFL, anti-GM3 antibodies and IL-18 in serum and cerebrospinal fluid (CSF) of patients with RRMS and PPMS, and to compare both forms of the disease with a control group consisting of patients with other neurological disorders (OND).
Methods: 25 patients with RRMS (39.5±12 y/o), 24 patients with PPMS (49.9±10.5 y/o) and 31 patients with OND (36 ±10,4 y/o) have been included in the study. Quantitative test kit based on sandwich ELISA has been used for NFL, anti-GM3 antibodies and IL-18 measurement. Kruskal-Wallis test for statistical analysis.
Results: The mean serum NFL concentration in PPMS (413,1 ±306,4 pg/ml) was significantly lower (p=0.012) in comparison with control group (657,2 ±175,5 pg/ml). The mean concentration of anti-GM3 Ab in CSF was significantly lower (p=0.002) in PPMS (2,6 ±0,9 ng/ml) in comparison with control group (3,6 ±1 ng/ml).In PPMS serum IL-18 level (285,8 ±100,4 pg/ml) was significantly higher (p=0.075) in comparison with control group (224,6 ±135,6 pg/ml). There was positive correlation in CSF between anti-GM3 Ab and IL-18 in all groups of patients. We did not found correlation between NFL, anti-GM3 Ab and IL-18 in CSF and age, duration of disease, EDSS, IgG level or IgG index.
Conclusions: Results demonstrate some immunological differences between PPMS and control group. There was no significantly statistical difference between NFL, anti-GM3 antibodies and IL-18 concentrations in serum and CSF of the two forms of MS.
Disclosure: Scientific grant from Biogen.
Piotr Iwanowski has no financial conflict of interest.
Jacek Losy has no financial conflict of interest.
Abstract: P1666
Type: LB Poster
Abstract Category: Late Breaking News
Background and goals: Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS) with an autoimmune reaction that cause distinct clinical courses.The immunopathological differences between relapsing-remitting (RRMS) and primary progressive multiple sclerosis (PPMS) are not fully explored.Neurofilament light polypeptide (NFL) is an intracellular protein supplying cytoskeleton of neurons. Axonal damage is associated with NFL degradation and secretion into the CSF.NFL levels in CSF probably reflects the degree of MS-associated neurodegeneration.Gangliosides constitute an axonal antigen and are also minor constituents of myelin. The synthesis of pathogenic autoantibodies to gangliosides may cause an injury to neuronal tissue and induce a complex local immune process. Interleukin 18 (IL-18) is an pro-inflammatory cytokine which is a costimulatory factor for production of IFN-gamma by T helper 1-type cells.
The aim of the study is to investigate the concentrations of NFL, anti-GM3 antibodies and IL-18 in serum and cerebrospinal fluid (CSF) of patients with RRMS and PPMS, and to compare both forms of the disease with a control group consisting of patients with other neurological disorders (OND).
Methods: 25 patients with RRMS (39.5±12 y/o), 24 patients with PPMS (49.9±10.5 y/o) and 31 patients with OND (36 ±10,4 y/o) have been included in the study. Quantitative test kit based on sandwich ELISA has been used for NFL, anti-GM3 antibodies and IL-18 measurement. Kruskal-Wallis test for statistical analysis.
Results: The mean serum NFL concentration in PPMS (413,1 ±306,4 pg/ml) was significantly lower (p=0.012) in comparison with control group (657,2 ±175,5 pg/ml). The mean concentration of anti-GM3 Ab in CSF was significantly lower (p=0.002) in PPMS (2,6 ±0,9 ng/ml) in comparison with control group (3,6 ±1 ng/ml).In PPMS serum IL-18 level (285,8 ±100,4 pg/ml) was significantly higher (p=0.075) in comparison with control group (224,6 ±135,6 pg/ml). There was positive correlation in CSF between anti-GM3 Ab and IL-18 in all groups of patients. We did not found correlation between NFL, anti-GM3 Ab and IL-18 in CSF and age, duration of disease, EDSS, IgG level or IgG index.
Conclusions: Results demonstrate some immunological differences between PPMS and control group. There was no significantly statistical difference between NFL, anti-GM3 antibodies and IL-18 concentrations in serum and CSF of the two forms of MS.
Disclosure: Scientific grant from Biogen.
Piotr Iwanowski has no financial conflict of interest.
Jacek Losy has no financial conflict of interest.