Contributions
Abstract: EP1443
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - Imaging
Introduction: Disturbances of the hypothalamo-pituitary (HPA) axis are supposed to be involved into pathogenesis of multiple sclerosis (MS). Moreover, the HPA connections with limbic system and frontal cortex can cause depression, frequently reported phenomenon associated with multiple sclerosis (MS).
Method: We focused on in-vivo evaluation of hypothalamic metabolism via 1H- MR spectroscopy (1H-MRS) in 33 MS patients with relapsing-remitting and secondary progressive disease, and 24 age and sex- related healthy individuals (CON). Concentrations of glutamate+glutamin (Glx), cholin (Cho), myoinositol (mIns), N-acetyl aspartate (NAA) expressed as ratio with creatine (Cr) and N-acetyl aspartate (NAA) were correlated with Simple Depressive-Severity Status Scale (SDSS). In MS patients relationship of metabolites with disease activity using RIO score and with Multiple Sclerosis Severity Scale (MSSS) was evaluated.
Results: Glutamate was the only metabolite correlated with MS activity (Glx/Cr with mRIO=0 vs Glx/Cr with mRIO>=1: p = 0.005 // Glx/NAA with mRIO=0 vs Glx/NAA with mRIO>=1: p = 0.00018. Mann-Whitney). We found also association of glutamate concentrations and MSSS (Glx/Cr: r=0.4, p=0.02, Glx/NAA: r=0.56, p=0.001 Spear). In MS patients depressive score correlated with increased glutamate concentrations (SDSS vs Glx/Cr: r = -0.33, p = 0.028, Glx/NAA: r = -0.33, p = 0.08 Spear).
In CON Glx correlated with age (Glx/Cr: r = -0.42, p = 0.048, Glx/NAA: r = -0.41, p = 0.056 Spearman), but a relationship of Glx and SDSS was not proved.
Conclusion: Glutamate-induced damage of hypothalamus reflects activity and also severity of the disease. Depression appears to be rather symptom of MS than comorbid disease. In healthy individuals we proved age-related changes of Glx. It is a first study revealing the relationship of depression, disease activity and hypothalamic metabolism in MS.
This work was supported by VEGA 1/0287/16.
Disclosure: Authors confirm that there are no known conflict of interest associated with this work. There has been no significant financial support for this work that could have influenced its outcome.
Ema Kantorova, Hubert Polacek, Michal Bittšanský, Eva Baranovičová, Petra Hnilicova, Daniel Čierny, Stefan Sivák, Vladimir Nosal, Kamil Zelenak, Dusan Dobrota, Egon Kurca have nothing to disclose
Abstract: EP1443
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - Imaging
Introduction: Disturbances of the hypothalamo-pituitary (HPA) axis are supposed to be involved into pathogenesis of multiple sclerosis (MS). Moreover, the HPA connections with limbic system and frontal cortex can cause depression, frequently reported phenomenon associated with multiple sclerosis (MS).
Method: We focused on in-vivo evaluation of hypothalamic metabolism via 1H- MR spectroscopy (1H-MRS) in 33 MS patients with relapsing-remitting and secondary progressive disease, and 24 age and sex- related healthy individuals (CON). Concentrations of glutamate+glutamin (Glx), cholin (Cho), myoinositol (mIns), N-acetyl aspartate (NAA) expressed as ratio with creatine (Cr) and N-acetyl aspartate (NAA) were correlated with Simple Depressive-Severity Status Scale (SDSS). In MS patients relationship of metabolites with disease activity using RIO score and with Multiple Sclerosis Severity Scale (MSSS) was evaluated.
Results: Glutamate was the only metabolite correlated with MS activity (Glx/Cr with mRIO=0 vs Glx/Cr with mRIO>=1: p = 0.005 // Glx/NAA with mRIO=0 vs Glx/NAA with mRIO>=1: p = 0.00018. Mann-Whitney). We found also association of glutamate concentrations and MSSS (Glx/Cr: r=0.4, p=0.02, Glx/NAA: r=0.56, p=0.001 Spear). In MS patients depressive score correlated with increased glutamate concentrations (SDSS vs Glx/Cr: r = -0.33, p = 0.028, Glx/NAA: r = -0.33, p = 0.08 Spear).
In CON Glx correlated with age (Glx/Cr: r = -0.42, p = 0.048, Glx/NAA: r = -0.41, p = 0.056 Spearman), but a relationship of Glx and SDSS was not proved.
Conclusion: Glutamate-induced damage of hypothalamus reflects activity and also severity of the disease. Depression appears to be rather symptom of MS than comorbid disease. In healthy individuals we proved age-related changes of Glx. It is a first study revealing the relationship of depression, disease activity and hypothalamic metabolism in MS.
This work was supported by VEGA 1/0287/16.
Disclosure: Authors confirm that there are no known conflict of interest associated with this work. There has been no significant financial support for this work that could have influenced its outcome.
Ema Kantorova, Hubert Polacek, Michal Bittšanský, Eva Baranovičová, Petra Hnilicova, Daniel Čierny, Stefan Sivák, Vladimir Nosal, Kamil Zelenak, Dusan Dobrota, Egon Kurca have nothing to disclose