Contributions
Abstract: EP1434
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - Environmental risk factors
Incidence of Multiple Sclerosis (MS) varies greatly with geographical location, being higher in western countries. Environmental air pollution has been associated with increased incidence and exacerbation of numerous respiratory, cardiovascular and rheumatologic diseases; however, little is known about the impact of air pollution on MS course. Here we investigated the relationship between air pollution, namely Particulate Matter 10 (PM10), and MS disease activity. 227 brain Magnetic Resonance Imaging (MRI), of which 77 (34%) showing contrast enhancing (CE) lesions, of 53 patients were considered. The mean PM10 level in 15 days prior MRI was significantly higher in patients with CE lesions compared with subjects without CE lesions (50,40 95%CI 44,70-56,26 vs 42,09 95%CI 38,47-45,60; f= 5,151, p=0,024), independently from time of the year, treatments and smoker-status. By Fluorescence-Activated Cell Sorting (FACS), we observed a significant correlation between mean PM10 levels and expression of CCR6 and PSGL1 on CD4+ and CD8+ T cells and LFA1 on CD4+ T cells. Moreover, in vitro treatment of Peripheral Blood Mononuclear Cells (PBMCs) with urban particulate matter led to increased expression of LFA1 and CCR6. Finally, urban particulate matter strongly induced secretion by monocyte derived Dendritic Cells (mdDCs) of Th17 polarizing IL6 and IL23 and, in mixed mdDC/PBMC cultures enhanced generation of IL17-producing T cells. We speculate that PM10 exposure in the lung may lead to MS exacerbation by inducing the production of autoreactive Th17 lymphocytes and by boosting their migratory capacity through the blood-brain barrier.
Disclosure: S. Villa: nothing to disclose
A. Cortese: nothing to disclose
L. Lova: nothing to disclose
P. Comoli: nothing to disclose
S. LaSalvia: nothing to disclose
G. Nosari: nothing to disclose
G. Mallucci: nothing to disclose
A. Romani: nothing to disclose
D. Franciotta: nothing to disclose
F. Gigli Berzolari: nothing to disclose
P. Borrelli: nothing to disclose
E. Volpe: nothing to disclose
S. Basso: nothing to disclose
I. Guido: nothing to disclose
G. Quartuccio: nothing to disclose
C. Cereda: nothing to disclose
V. Bollati: nothing to disclose
L. Battistini: nothing to disclose
R. Bergamaschi: nothing to disclose
Abstract: EP1434
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - Environmental risk factors
Incidence of Multiple Sclerosis (MS) varies greatly with geographical location, being higher in western countries. Environmental air pollution has been associated with increased incidence and exacerbation of numerous respiratory, cardiovascular and rheumatologic diseases; however, little is known about the impact of air pollution on MS course. Here we investigated the relationship between air pollution, namely Particulate Matter 10 (PM10), and MS disease activity. 227 brain Magnetic Resonance Imaging (MRI), of which 77 (34%) showing contrast enhancing (CE) lesions, of 53 patients were considered. The mean PM10 level in 15 days prior MRI was significantly higher in patients with CE lesions compared with subjects without CE lesions (50,40 95%CI 44,70-56,26 vs 42,09 95%CI 38,47-45,60; f= 5,151, p=0,024), independently from time of the year, treatments and smoker-status. By Fluorescence-Activated Cell Sorting (FACS), we observed a significant correlation between mean PM10 levels and expression of CCR6 and PSGL1 on CD4+ and CD8+ T cells and LFA1 on CD4+ T cells. Moreover, in vitro treatment of Peripheral Blood Mononuclear Cells (PBMCs) with urban particulate matter led to increased expression of LFA1 and CCR6. Finally, urban particulate matter strongly induced secretion by monocyte derived Dendritic Cells (mdDCs) of Th17 polarizing IL6 and IL23 and, in mixed mdDC/PBMC cultures enhanced generation of IL17-producing T cells. We speculate that PM10 exposure in the lung may lead to MS exacerbation by inducing the production of autoreactive Th17 lymphocytes and by boosting their migratory capacity through the blood-brain barrier.
Disclosure: S. Villa: nothing to disclose
A. Cortese: nothing to disclose
L. Lova: nothing to disclose
P. Comoli: nothing to disclose
S. LaSalvia: nothing to disclose
G. Nosari: nothing to disclose
G. Mallucci: nothing to disclose
A. Romani: nothing to disclose
D. Franciotta: nothing to disclose
F. Gigli Berzolari: nothing to disclose
P. Borrelli: nothing to disclose
E. Volpe: nothing to disclose
S. Basso: nothing to disclose
I. Guido: nothing to disclose
G. Quartuccio: nothing to disclose
C. Cereda: nothing to disclose
V. Bollati: nothing to disclose
L. Battistini: nothing to disclose
R. Bergamaschi: nothing to disclose