Contributions
Abstract: EP1428
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - Immunology
Background: Myelin lipids are believed to be implicated in the immunopathogenesis of multiple sclerosis (MS). However, mechanisms of lipid autoreactivity in MS are not fully characterized. In this study we investigated cytokines/chemokines production in peripheral blood mononuclear cells (PBMC) after stimulation with myelin lipids in patients with multiple sclerosis compared to healthy volunteers.
Materials and methods: The study included 29 MS patients and 20 age and sex matched healthy controls (HC). PBMC were isolated and cultured with myelin lipids (phosphatydolcholine, galactocerebrosides, sulfatides, gangliosides GM4, GM2, GD1a) during 18 h. Cytokines/chemokines concentrations were measured in PBMC media after stimulation with myelin lipids and in intact PBMC media. Following cytokines/chemokines levels were determined: IL-1ra (interleukin-1 receptor antagonist), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, Eotaxin, bFGF (basic fibroblast growth factor), G-CSF (granulocyte-colony stimulating factor), GM-CSF (granulocyte-macrophage colony-stimulating factor), IFN-γ (interferon gamma), IP-10 (interferon gamma-induced protein 10), MCP-1 (monocyte chemoattractant protein-1), MIP-1α and MIP-1β (macrophage inflammatory proteins 1-alpha and 1-beta), PDGF (platelet-derived growth factor), RANTES (regulated on activation, normal T cell expressed and secreted), TNFα (tumor necrosis factor alpha), VEGF (vascular endothelial growth factor).
Results: Higher concentrations of IL1ra, IL6, IL8, IL17A (p< 0,05), MIP1a, MIP1b, IFNg, TNFa, eotaxin, IL9 (p< 0,05), PDGF, G-CSF, GM-CSF, VEGF were observed in intact cell media of MS patients. Higher concentrations of IL1ra, IL6, G-CSF, PDGF, VEGF were found in PBMC media of MS patients after incubation with myelin lipids. There was a decrease in IL-10, PDGF, G-CSF and VEGF production after stimulation with all of the tested lipids in MS patients that was more pronounced in patients with more severe disease. In HC PBMC media an increase in IL6, IL17A and MIP1a concentrations and a decrease in IL-10 and PDGF concentrations were found.
Conclusion: Myelin lipids induce different cytokine secretion profiles in MS patients and healthy controls.
Disclosure: Ivanova M: nothing to disclose; Tukhvatulin A.: nothing to disclose; Dzharullaeva A.: nothing to disclose; Logunov D.: nothing to disclose; Zakharova M.: nothing to disclose
Abstract: EP1428
Type: ePoster
Abstract Category: Pathology and pathogenesis of MS - Immunology
Background: Myelin lipids are believed to be implicated in the immunopathogenesis of multiple sclerosis (MS). However, mechanisms of lipid autoreactivity in MS are not fully characterized. In this study we investigated cytokines/chemokines production in peripheral blood mononuclear cells (PBMC) after stimulation with myelin lipids in patients with multiple sclerosis compared to healthy volunteers.
Materials and methods: The study included 29 MS patients and 20 age and sex matched healthy controls (HC). PBMC were isolated and cultured with myelin lipids (phosphatydolcholine, galactocerebrosides, sulfatides, gangliosides GM4, GM2, GD1a) during 18 h. Cytokines/chemokines concentrations were measured in PBMC media after stimulation with myelin lipids and in intact PBMC media. Following cytokines/chemokines levels were determined: IL-1ra (interleukin-1 receptor antagonist), IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, Eotaxin, bFGF (basic fibroblast growth factor), G-CSF (granulocyte-colony stimulating factor), GM-CSF (granulocyte-macrophage colony-stimulating factor), IFN-γ (interferon gamma), IP-10 (interferon gamma-induced protein 10), MCP-1 (monocyte chemoattractant protein-1), MIP-1α and MIP-1β (macrophage inflammatory proteins 1-alpha and 1-beta), PDGF (platelet-derived growth factor), RANTES (regulated on activation, normal T cell expressed and secreted), TNFα (tumor necrosis factor alpha), VEGF (vascular endothelial growth factor).
Results: Higher concentrations of IL1ra, IL6, IL8, IL17A (p< 0,05), MIP1a, MIP1b, IFNg, TNFa, eotaxin, IL9 (p< 0,05), PDGF, G-CSF, GM-CSF, VEGF were observed in intact cell media of MS patients. Higher concentrations of IL1ra, IL6, G-CSF, PDGF, VEGF were found in PBMC media of MS patients after incubation with myelin lipids. There was a decrease in IL-10, PDGF, G-CSF and VEGF production after stimulation with all of the tested lipids in MS patients that was more pronounced in patients with more severe disease. In HC PBMC media an increase in IL6, IL17A and MIP1a concentrations and a decrease in IL-10 and PDGF concentrations were found.
Conclusion: Myelin lipids induce different cytokine secretion profiles in MS patients and healthy controls.
Disclosure: Ivanova M: nothing to disclose; Tukhvatulin A.: nothing to disclose; Dzharullaeva A.: nothing to disclose; Logunov D.: nothing to disclose; Zakharova M.: nothing to disclose