Contributions
Abstract: EP1336
Type: ePoster
Abstract Category: Clinical aspects of MS - Diagnosis and differential diagnosis
Objectives: HLA-DR and HLA-DQ alleles within the Human Leucocyte Antigen (HLA) class II region on chromosome 6p21 are known to play a significant role in several autoimmune diseases including Neuromyelitis Optica(NMO) and Multiple Sclerosis (MS).Objective of our study is to know HLA alleles and their association with NMO and MS.
Methods: We have genotyped for DR and DQ loci by sequence-specific polymerase chain reaction(PCR)-based typing in 38 patients with NMO, 16 patients with MS and 86 healthy controls.
Results: We found an association of HLA-DRB1*10 alleles (P=0.02, Odds ratio 2.86, 95% CI 1.20-6.80) with NMO antibody positive patients as compared to HLA-DRB1*05 and HLA-DRB1*03 in Japanese and Caucasian / Indian patients respectively. Additionally HLA-DRB1*04/HLA-DRB1*10 genotype showed significant association with NMO. A significant protective role was found for HLA-DRB1*14/HLA-DRB1*15 genotype (P=0.03) with NMO patients. But we could not found any significant association of HLA-DQB1 alleles and genotypes with NMO. The haplotype carriers of DRB1*03-DQB1*05, DRB1*15-DQB1*03 and DRB1*16-DQB1*06 showed positive association while the haplotype carriers of DRB1*14-DQB1*05 and DRB1*15-DQB1*06 showed negative association with NMO.We found a positive association between MS susceptibility and the DRB1*15 alleles.
Conclusion: This gives us the evidence that apart from distinct alleles, different haplotype combinations have also been associated with NMO.
Disclosure: Rakesh Kathgave :Nothing to disclose
Abstract: EP1336
Type: ePoster
Abstract Category: Clinical aspects of MS - Diagnosis and differential diagnosis
Objectives: HLA-DR and HLA-DQ alleles within the Human Leucocyte Antigen (HLA) class II region on chromosome 6p21 are known to play a significant role in several autoimmune diseases including Neuromyelitis Optica(NMO) and Multiple Sclerosis (MS).Objective of our study is to know HLA alleles and their association with NMO and MS.
Methods: We have genotyped for DR and DQ loci by sequence-specific polymerase chain reaction(PCR)-based typing in 38 patients with NMO, 16 patients with MS and 86 healthy controls.
Results: We found an association of HLA-DRB1*10 alleles (P=0.02, Odds ratio 2.86, 95% CI 1.20-6.80) with NMO antibody positive patients as compared to HLA-DRB1*05 and HLA-DRB1*03 in Japanese and Caucasian / Indian patients respectively. Additionally HLA-DRB1*04/HLA-DRB1*10 genotype showed significant association with NMO. A significant protective role was found for HLA-DRB1*14/HLA-DRB1*15 genotype (P=0.03) with NMO patients. But we could not found any significant association of HLA-DQB1 alleles and genotypes with NMO. The haplotype carriers of DRB1*03-DQB1*05, DRB1*15-DQB1*03 and DRB1*16-DQB1*06 showed positive association while the haplotype carriers of DRB1*14-DQB1*05 and DRB1*15-DQB1*06 showed negative association with NMO.We found a positive association between MS susceptibility and the DRB1*15 alleles.
Conclusion: This gives us the evidence that apart from distinct alleles, different haplotype combinations have also been associated with NMO.
Disclosure: Rakesh Kathgave :Nothing to disclose